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KMID : 0363620110320030010
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Journal of Korean Oriental Medicine
2011 Volume.32 No. 3 p.10 ~ p.17
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Broussonetia kazinoki Siebold stimulates immune response in ovalbumin-immunized mice
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Jung Da-Young
Ha Hye-Kyung
Lee Ho-Young
Lee Jin-Ah
Jeong Seung-Il
Choi Young-Jae
Shin Hyeun-Kyoo
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Abstract
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Objective: To evaluate the immune-stimulatory potential of extracts of Broussonetia kazinoki Siebold (BK) on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice.
Material and Methods: C57BL/6 mice were immunized intraperitoneally with OVA/alum () on days 1, 8, and 15. BK (100, 300 or 1000 mg/kg) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Further, the effects of BK on expression of cytokine mRNA in OVA-immunized mice splenocytes were evaluated by RT-PCR analysis.
Results: BK significantly enhanced OVA-, LPS-, and Con A-induced splenocyte proliferation in OVA-immunized mice (p<0.01). BK also significantly enhanced total IgM and OVA-specific IgG1 levels in plasma compared with the OVA control group. Moreover, BK up-regulated significantly the expression of mRNA level of IL-2 and IFN- in splenocytes.
Conclusions: BK has immune-stimulating activity in an OVA-immunized mouse model system, enhancing the Th1 immune response. BK showed no cytotoxicity in this system, suggesting that BK may be a safe and effective adjuvant in humans.
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KEYWORD
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Broussonetia kazinoki Siebold, OVA, splenocyte proliferation, antibody, adjuvant, immune response
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